Microbial flora of neonatal mouse intestinal mucosal T cells and bone marrow cellsHite  Publish time :2017-08-10
Published an article by Willams AM, Probert CS, Stepankova R et al., Scholar of the Department of Clinical Sciences at the University of Bristol, UK, published in the Scientific Journal "Immunology", Vol. 119, No. 4, 2006. Microflora on the neonatal development of gut mucosal T cells and myeloid cells in the mouse "(translated into" Microbial flora on the early development of intestinal mucosal T cells and bone marrow cells in mice "). This paper describes the effect of microbial flora on immune cells in neonatal mouse mucosa. Now the article abstract is translated as follows:
The colonization of symbiotic flora in the early stages of neonatal life may have a profound effect on the development of small intestinal lymphoid and post-biotic immune responses. We clearly defined the effects of intestinal T cell phenotype and the development of intestinal immune function in mice by intestinal flora. The intestinal T cells were phenotypically classified and quantified by immunohistochemistry, routine (CV), sterile (GF) and traditional sterile (GF / CV) neonatal mice. When CV and GF mice were born, mucosal address cell adhesion molecule-1 (MAdCAM-1) was expressed on the mucosal vessels, and CVs were more distributed in the small intestine than GF The growth has not changed. Was less expressed in the colon; after weaning, its distribution was limited, and there was no difference between CV and GF mice. CD3 (+) beta (7) (+) cells showed a similar amount in CV and GF intestinal tract at birth. In CV mice, they were CD62L (-) and were accompanied by further development of CD3 (+) beta (7) (+) CD62L (-) T cells, but in GF and GF / CV intestine, The number of CD62L expressed has not changed. After weaning, the number of IELs increased in the large intestine and small intestine of CV-type mice, but the development of the intestinal tract was delayed. In the small intestine of the GF, macrophages are most expressed from the beginning of birth, but until the 16th day of birth, dendritic cells are not developed. Thus, at the time of birth through the beta-MadCAM interaction, fetal derived T cells dormant in the intestinal lamina propria. Their activation status depends on the condition of the microbial flora, and if there are no symbiotic flora, they remain the original ecology. We can assume that these cells regulate the antigenic response of the developing mucosal T cell pool.
(Mucosal addressin cell adhesion molecule 1, MAdCAM-1) belongs to the family of immunoglobulin superfamily, which is mainly expressed in the intestinal mucosa lymphoid tissue or lamina propria vascular endothelium, which is associated with lymphocytes On the homing receptor molecules, mediate the lymphocytes to the mucosal site of the homing to participate in the body's local immunity.